Psilocybin content differed across the five tested Psilocybe cubensis samples, with measured psilocybin ranging from 6.134 to 9.0435 mg/g of dry weight. Combined psilocybin and psilocin ranged from 0.649% to 0.956%. These figures are single-sample laboratory results for five strains in a larger genetic library. They show measured variation across the samples and do not establish a fixed potency range for each strain.
Psilocybe cubensis strain potency comparison
The comparison uses dried mushroom fruiting-body samples analyzed by ACS Laboratory. Samples were collected and analyzed between September 21 and September 26, 2023. Each row represents one tested sample from one named strain.
| Strain sample | Psilocybin mg/g | Psilocybin dry weight | Psilocin mg/g | Psilocin dry weight | Combined psilocybin and psilocin | Total measured alkaloids | Moisture |
|---|---|---|---|---|---|---|---|
| Sacred Sun 2-P2 | 9.0435 | 0.904% | 0.522 | 0.0522% | 0.956% | 1.0213% | 6.12% |
| Old Dirty PE 1-P1 | 8.118 | 0.812% | 0.130 | 0.0130% | 0.825% | 0.895% | 6.50% |
| Blue Meanie 4-P4 | 6.991 | 0.699% | 0.275 | 0.0275% | 0.727% | 0.823% | 6.45% |
| DC MAK 5-P5 | 6.322 | 0.632% | 0.538 | 0.0538% | 0.686% | 0.745% | 5.88% |
| Hawaiian 3-P3 | 6.134 | 0.613% | 0.364 | 0.0364% | 0.649% | 0.697% | 5.94% |
The table is ordered by combined psilocybin and psilocin. ACS reported concentrations on a dry-weight basis. Total measured alkaloids include psilocybin, psilocin, baeocystin, norbaeocystin, norpsilocin, aeruginascin and 4-Hydroxy-TMT.
Measured potency range across the five samples
The public dataset produced the following observed ranges.
| Measurement | Lowest result | Highest result | Observed range across samples |
|---|---|---|---|
| Psilocybin | 6.134 mg/g | 9.0435 mg/g | 6.134 to 9.0435 mg/g |
| Psilocybin dry weight | 0.613% | 0.904% | 0.613% to 0.904% |
| Psilocin | 0.130 mg/g | 0.538 mg/g | 0.130 to 0.538 mg/g |
| Psilocin dry weight | 0.0130% | 0.0538% | 0.0130% to 0.0538% |
| Combined psilocybin and psilocin | 0.649% | 0.956% | 0.649% to 0.956% |
| Total measured alkaloids | 0.697% | 1.0213% | 0.697% to 1.0213% |
Sacred Sun 2-P2 produced the highest psilocybin result and the highest combined result in this set. Hawaiian 3-P3 produced the lowest figures for both measurements. The Sacred Sun combined concentration was about 47% higher than the Hawaiian result.
This comparison describes the five samples submitted for testing. Repeated samples from several harvests would be needed to calculate a strain-specific average, distribution and tested range.
What the five strain results show
Sacred Sun 2-P2 had the highest combined result
Sacred Sun contained 9.0435 mg/g of psilocybin and 0.522 mg/g of psilocin. Its combined concentration reached 0.956% of dry weight. Total measured alkaloids reached 1.0213%.
The sample also contained 0.488 mg/g of baeocystin, 0.061 mg/g of norbaeocystin, 0.0555 mg/g of norpsilocin, 0.0289 mg/g of aeruginascin and 0.0175 mg/g of 4-Hydroxy-TMT. Its 4-Hydroxy-TMT result was the highest among the five samples.
Old Dirty PE 1-P1 had high psilocybin and low psilocin
Old Dirty PE contained 8.118 mg/g of psilocybin, the second-highest result in the table. Its psilocin result was 0.130 mg/g, the lowest of the five samples. Combined psilocybin and psilocin reached 0.825%. Total measured alkaloids reached 0.895%.
This pattern shows why total psilocybin alone does not describe the entire alkaloid profile. Two samples with similar total potency can distribute their measured compounds in different ratios.
Blue Meanie 4-P4 had the highest baeocystin result
Blue Meanie contained 6.991 mg/g of psilocybin and 0.275 mg/g of psilocin. Its combined concentration was 0.727%, placing it third among the five tested samples.
The sample contained 0.800 mg/g of baeocystin, the highest baeocystin result in the set. Its norbaeocystin result of 0.102 mg/g was also the highest. Those figures gave Blue Meanie a total measured alkaloid concentration of 0.823%.
DC MAK 5-P5 had the highest psilocin result
DC MAK contained 6.322 mg/g of psilocybin and 0.538 mg/g of psilocin. Its psilocin value was the highest in the set, even though its psilocybin value ranked fourth. Combined psilocybin and psilocin reached 0.686%.
DC MAK also produced the highest norpsilocin result at 0.0678 mg/g. The sample shows how individual alkaloid rankings can differ from the psilocybin ranking.
Hawaiian 3-P3 had the lowest combined result
Hawaiian contained 6.134 mg/g of psilocybin and 0.364 mg/g of psilocin. Its combined concentration was 0.649%. Total measured alkaloids reached 0.697%.
The result identifies the concentration in this submitted sample. Cultivation records and repeated chemical analysis are needed before you can describe typical performance for the wider genetic line.
Minor alkaloid profiles also differed
The five ACS reports measured five compounds in addition to psilocybin and psilocin.
| Strain sample | Baeocystin mg/g | Norbaeocystin mg/g | Norpsilocin mg/g | Aeruginascin mg/g | 4-Hydroxy-TMT mg/g |
|---|---|---|---|---|---|
| Sacred Sun 2-P2 | 0.488 | 0.0610 | 0.0555 | 0.0289 | 0.0175 |
| Old Dirty PE 1-P1 | 0.566 | 0.0781 | 0.0103 | 0.0398 | 0.00439 |
| Blue Meanie 4-P4 | 0.800 | 0.1020 | 0.0329 | 0.0249 | 0.00545 |
| DC MAK 5-P5 | 0.450 | 0.0537 | 0.0678 | 0.0148 | 0.0104 |
| Hawaiian 3-P3 | 0.360 | 0.0439 | 0.0350 | 0.0243 | 0.00829 |
You can see different chemical profiles even within a small group of P. cubensis samples. Blue Meanie led the set in baeocystin and norbaeocystin. DC MAK led in norpsilocin. Old Dirty PE led in aeruginascin. Sacred Sun led in 4-Hydroxy-TMT.
These profile differences do not establish distinct subjective or clinical effects. Human research has not defined the contribution of each minor alkaloid at these concentrations. The results support chemical comparison and future study.
How to read psilocybin percentages and mg/g
A dry-weight percentage tells you how much of a dried sample consists of a measured compound. A concentration of 1% equals 10 mg/g. Sacred Sun measured 0.904% psilocybin, which corresponds to about 9.04 mg/g.
You should keep three units separate when reading a report.
- mg/g gives milligrams of a compound per gram of dried sample
- Dry-weight percentage gives the same concentration as a percentage
- Milligrams per package gives the total amount in the tested package
Package totals depend on package weight. They are less useful for comparing samples of different sizes. The mg/g and dry-weight percentage columns allow a direct concentration comparison.
The combined psilocybin and psilocin figure in these reports is a simple sum of their dry-weight percentages. Psilocybin and psilocin have different molecular weights and pharmacology. The combined percentage provides a useful composition figure. It should not be treated as a complete measure of expected human response.
Strain name alone cannot predict potency
A Psilocybe cubensis strain name identifies a cultivated line or market label. Naming practices are not standardized across all cultivators. Two cultures sold under the same familiar name may have different genetic histories, selection records and chemical profiles.
Even a verified genetic line can produce variable alkaloid concentrations. Genetics affect biosynthetic capacity. Cultivation conditions affect expression. Relevant variables include substrate, temperature, humidity, air exchange, harvest timing, flush number, fruiting-body part, drying and storage.
A controlled 2026 study of 14 P. cubensis strains found more than sevenfold differences in total tryptamines across strains grown under the same conditions. The researchers also tested five individual fruiting bodies from selected strains. Within-strain coefficients of variation ranged from about 13% to 23%.
An earlier study of psilocybin-producing mushroom collections also found wide differences in tryptamine concentrations across species and samples. Together, these results show why you need chemical data from the tested batch.
Drying, moisture and storage affect comparison
The five ACS samples had moisture levels from 5.88% to 6.50%. This narrow span supports a more direct dry-weight comparison because the samples were similarly dried.
Moisture still needs to appear in the data. A wetter sample contains more water per gram, which can lower an as-received concentration. Dry-weight reporting corrects for this effect and makes samples easier to compare.
Post-harvest handling can also change measured alkaloids. A Psilocybe cubensis stability study found changes after tissue damage, drying, grinding and storage. Psilocybin, psilocin and minor compounds did not change at identical rates under every condition.
For repeated strain testing, you need consistent harvest, drying, storage and sampling procedures. A genetic label linked to cultivation metadata gives you a stronger dataset than a name linked to one isolated result.
Conclusion
To create a true potency range for one strain, you need several representative samples from separate fruiting bodies, harvests and batches. The test plan should use the same analytical method and report results on the same basis.
A useful strain dataset records the following fields.
- Verified genetic identifier
- Cultivation batch and harvest date
- Substrate and controlled growing conditions
- Flush number and fruiting-body part
- Drying and storage method
- Moisture at testing
- Psilocybin, psilocin and minor alkaloids in mg/g
- Mean, median, minimum, maximum and standard deviation
Single-sample results remain useful as measured observations. Their role is to establish what the laboratory found in that submitted material. Repeated testing shows how a strain performs across time and production conditions.
As you compare strain potency data, we at Rose Hill Life Sciences work as a psychedelic research organization focused on the production and research of Psilocybe cubensis. We maintain a proprietary library of more than 50 genetic strains and link strain identity with cultivation and chemical records. Our Massachusetts research work sits at the intersection of psychedelic science and therapeutic integration, with ACS testing used to document the five public strain samples presented here.
Disclaimer: The information in this article is for educational and informational purposes only and does not constitute medical advice.
